This toolkit can be easily used to create a variant allele summary plot and accompanying table of counts. CrispRVariants allows users to detect problems and filter sequences appropriately before estimating mutation efficiency. Individual variant alleles from either traditional Sanger sequencing or high-throughput CRISPR-Cas9 mutagenesis sequencing experiments can be quantified and visualized. Off spotter helps in the design of optimal "guide" RNAs (gRNAs) by providing several protospacer adjacent motif (PAM) choices, a run-time definition of the seed and of the allowed number of mismatches.ĬrispRVariants resolves and localizes individual mutant alleles with respect to the endonuclease cut site.
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